The König lab combines biochemistry with high-throughput approaches to obtain mechanistic insights into regulatory processes in the RNA world. Regulation is achieved by the cooperative action of RNA-binding proteins (RBPs) which recognise RNA sequences in the pre-mRNA and modulate the activity of cellular machineries such as the ribosome and the spliceosome. In order to unravel this complexity, we integrate in vivo and in vitro RNA binding maps (iCLIP) with functional readouts and targeted reporters.
We participate in the FOR2333 Research Unit with the following project:
Project 1: Together with the Niessing and Zarnack labs, we investigate how RNA localisation elements (LEs) are specifically recognized by the SHE machinery during mRNA transport in yeast. Using transcriptome-wide in vitro and in vivo RBP binding maps, we will address which LEs are recognized by the SHE machinery in the cytoplasm and how SHE binding changes during nucleo-cytoplasmic remodeling of the transport complexes. Our combined approach will enable us to disentangle the combinatorial contribution of multiple proteins to SHE binding and mRNA localization this important model system.