Inga Loedige is interested in TRIM-NHL proteins, a family of conserved developmental regulators. During her post-doctoral work in Gunter Meisters Lab, Inga showed that the NHL domain, the defining feature of TRIM-NHL proteins, is an RNA-binding domain and that TRIM-NHL proteins bind mRNAs to control gene expression post-transcriptional.
In her current work, which is funded be a DFG grant supporting her own position, Inga aims to reveal how the two closely related mammalian TRIM-NHL proteins Trim2 and Trim3 regulate gene expression in neurons. She combines genome-wide approaches, biochemistry and cell-based assays using motor neurons differentiated from mouse embryonic stem cells in culture as a model system.
IRI Life Sciences, Humboldt University, Philippstr. 13, 10115, Berlin, Germany
Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin, Germany
Reichardt I, Bonnay F, Steinmann V, Loedige I, Burkard TR, Meister G, Knoblich JA. The tumor suppressor Brat controls neuronal stem cell lineages by inhibiting Deadpan and Zelda. EMBO Rep. 19, 102-117 (2018).
Loedige I, Jakob L, Treiber T, Ray D, Stotz M, Treiber N, Hennig J, Cook KB, Morris Q, Hughes TR, Engelmann JC, Krahn MP, Meister G. The Crystal Structure of the NHL Domain in Complex with RNA Reveals the Molecular Basis of Drosophila Brain-Tumor-Mediated Gene Regulation. Cell Rep 13, 1206-20 (2015).
Loedige I, Stotz M, Qamar S, Kramer K, Hennig J, Schubert T, Löffler P, Längst G, Merkl R, Urlaub H, Meister G. The NHL domain of BRAT is an RNA-binding domain that directly contacts the hunchback mRNA for regulation. Genes Dev 28, 749-64 (2014).
Loedige I, Gaidatzi D, Sack R, Meister G, Filipowicz W. The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function. Nucleic Acids Res 41, 518-32 (2013).
Krol J, Loedige I, Filipowicz W. The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Genet.11, 597-610 (2010). Review.